Simultaneous identification and qualitative determination of hydrocyanic acid and phosphine by gas chromatography with nitrogen-phosphorus detector and Headspace autosampler (hs-gc-npd) in biological fluids
DOI:
https://doi.org/10.16925/cf.v3i1.1188Keywords:
cyanide, gas chromatography, phosphine, simultaneous identificationAbstract
Introduction: This article described a simple, rapid, sensitive methodology for the identification and qualitative determination of phosphine and hydrocyanic cyanide in biological (i.e., blood and stomach contents) and non-biological samples by gas chromatography (gc). Methodology: The foregoing was carried out by a selective nitrogen-phosphorus detector (npd) and Headspace (hs) volatile autosampler using acetonitrile (acn) as internal standard (istd). The procedure involves taking and pouring into a vial 1 mL of the sample, which is added sulfuric acid. The vial is tightly sealed and subjected to incubation at 70 °C for 5 minutes. Finally, 1 mL of gas phase is injected into the gas chromatograph. Results: The limits of detection and quantification found for both phosphine and hydrocyanic acid were 0.04 mg/L and 0.2 mg/L, respectively. Conclusions: A chromatographic method was implemented and validated to allow simultaneous identification and quantitative determination of hydrocyanic acid and phosphine in biological samples such as blood and stomach contents.
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