Validation of detection method prostatic specific antigen (PSA) by inmunocromatrografyc (Orgenics) applied to seminal liquid in stain dry and swabs
DOI:
https://doi.org/10.16925/cf.v1i3.1385Keywords:
Forensic evidences, Immunocromatografy, Prostate Specific Antigen PSA, semenAbstract
The Prostate Specific Antigen (PSA) generally is used quantitatively to detect and to watch over the development of prostate of cancer by serum levels of this protein, but also is found in high concentrations in semen. Different sensitive, reproducible and simple methods have been developed for the analysis of the presence of PSA including inmunocromatografy techniques. The procedures most common in the forensic detection of semen have focused in the detection of spermatozoa, inmunoelectroforetic activity of acid phosphatase and methods for the PSA detection. Although these methods has been used by many years, but some problems are associated with each method. The objective of this investigation was to validate the inmunocromatografy test in the detection of PSA in forensic evidences. Different dilutions of semen standar (100.000 ng/ml of PSA) were made, determining the low limit detection in 10 ng/ml. Positive results were obtained since concentrations of 500ng/ml in stains and swabs. The performance of the test was evaluated in the presence of interferences, such as blood, male and female urine, maternal milk, vaginal secretions, feces and saliva. It shows positive results in samples of blood and male urine with prostatic cancer. We reported a sensitivity of 91.6 % and a specificity of 100 %. These results suggest that this presumtive test can be usefull as preliminar test to determinate presence of semen in forensic samples.
Author Biographies
Sandra Liliana Cifuentes, Instituto Nacional de Medicina Legal y Ciencias Forenses
Bacterióloga
Pedro Vargas Pinilla, Pontificia Universidad Javeriana
Biólogo
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