Validación de un método de análisis de cocaína, opiáceos y canabinoides mediante el análisis de pelo por GC/MSD para determinar consumo crónico
DOI:
https://doi.org/10.60007/antistio.4765Keywords:
hair, GC/MS, cocaine, opiates, canabinoides, validationAbstract
An analytical methodology was developed and fully validated to calculate the chronic consume of cocaine, opiates cannabinoids and their metabolites in hair samples, by using d-3,6 MAM-d3 cocaine and THC-COOH-d3 as inner standards. The method comprise a double liquid-liquid extraction, with dichloromethane and Hexane/Ethyl acetate. All substances were detected as their trimethysilyl derivatives (BSTFA/TMCs) in order to increase chromatographic sensitivity and subsequent chromatographic analysis with capillary column HP-5MS de 30 m* 0.25 mm y 0.25 µm. The methodology was selective, linear, precise and accurate. The method was linear over investigated range (one - ten µg/mg) with an excellent intra-assay and inter-assay precision (relative standard deviations, RSD < 15%). Limits of quantification were between 0,1 ng y 7,0 ng/mg and recovery percentages were between 70% y 92%.
Author Biographies
Isabel Riveros Toledo, Instituto Nacional de Medicina Legal y Ciencias Forenses. Bogotá, Colombia
Laboratorio de Toxicología- Regional Bogotá. Instituto Nacional de Medicina Legal y Ciencias Forenses. Bogotá, Colombia.
Jorge Ariel Martínez Ramírez, Universidad Nacional de Colombia. Bogotá, Colombia
Universidad Nacional de Colombia- Departamento de Farmacia. Bogotá, Colombia.
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